Ontology highlight
ABSTRACT:
SUBMITTER: Arola HO
PROVIDER: S-EPMC5408219 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Arola Henri O HO Tullila Antti A Nathanail Alexis V AV Nevanen Tarja K TK
Toxins 20170420 4
We developed an HT-2 toxin-specific simple ELISA format with a positive read-out. The assay is based on an anti-immune complex (IC) scFv antibody fragment, which is genetically fused with alkaline phosphatase (AP). The anti-IC antibody specifically recognizes the IC between a primary anti-HT-2 toxin Fab fragment and an HT-2 toxin molecule. In the IC ELISA format, the sample is added together with the scFv-AP antibody to the ELISA plate coated with the primary antibody. After 15 min of incubation ...[more]