Unknown

Dataset Information

0

Deep Sequencing Analysis of RNAs from Citrus Plants Grown in a Citrus Sudden Death-Affected Area Reveals Diverse Known and Putative Novel Viruses.


ABSTRACT: Citrus sudden death (CSD) has caused the death of approximately four million orange trees in a very important citrus region in Brazil. Although its etiology is still not completely clear, symptoms and distribution of affected plants indicate a viral disease. In a search for viruses associated with CSD, we have performed a comparative high-throughput sequencing analysis of the transcriptome and small RNAs from CSD-symptomatic and -asymptomatic plants using the Illumina platform. The data revealed mixed infections that included Citrus tristeza virus (CTV) as the most predominant virus, followed by the Citrus sudden death-associated virus (CSDaV), Citrus endogenous pararetrovirus (CitPRV) and two putative novel viruses tentatively named Citrus jingmen-like virus (CJLV), and Citrus virga-like virus (CVLV). The deep sequencing analyses were sensitive enough to differentiate two genotypes of both viruses previously associated with CSD-affected plants: CTV and CSDaV. Our data also showed a putative association of the CSD-symptomatic plants with a specific CSDaV genotype and a likely association with CitPRV as well, whereas the two putative novel viruses showed to be more associated with CSD-asymptomatic plants. This is the first high-throughput sequencing-based study of the viral sequences present in CSD-affected citrus plants, and generated valuable information for further CSD studies.

SUBMITTER: Matsumura EE 

PROVIDER: S-EPMC5408698 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Deep Sequencing Analysis of RNAs from Citrus Plants Grown in a Citrus Sudden Death-Affected Area Reveals Diverse Known and Putative Novel Viruses.

Matsumura Emilyn E EE   Coletta-Filho Helvecio D HD   Nouri Shahideh S   Falk Bryce W BW   Nerva Luca L   Oliveira Tiago S TS   Dorta Silvia O SO   Machado Marcos A MA  

Viruses 20170424 4


Citrus sudden death (CSD) has caused the death of approximately four million orange trees in a very important citrus region in Brazil. Although its etiology is still not completely clear, symptoms and distribution of affected plants indicate a viral disease. In a search for viruses associated with CSD, we have performed a comparative high-throughput sequencing analysis of the transcriptome and small RNAs from CSD-symptomatic and -asymptomatic plants using the Illumina platform. The data revealed  ...[more]

Similar Datasets

| S-EPMC5192391 | biostudies-literature
| S-EPMC8216279 | biostudies-literature
| S-EPMC7157637 | biostudies-literature
| S-EPMC3486453 | biostudies-literature
| S-EPMC3430378 | biostudies-literature
| S-EPMC10676361 | biostudies-literature
| S-EPMC9657546 | biostudies-literature
| S-EPMC9941208 | biostudies-literature
| S-EPMC10747610 | biostudies-literature
| S-EPMC3794751 | biostudies-literature