Ontology highlight
ABSTRACT:
SUBMITTER: Lisi GP
PROVIDER: S-EPMC5410785 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Lisi George P GP East Kyle W KW Batista Victor S VS Loria J Patrick JP
Proceedings of the National Academy of Sciences of the United States of America 20170410 17
Imidazole glycerol phosphate synthase (IGPS) is a V-type allosteric enzyme, meaning that its catalytic rate is critically dependent on activation by its allosteric ligand, <i>N'</i>-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide (PRFAR). The allosteric mechanism of IGPS is reliant on millisecond conformational motions for efficient catalysis. We engineered four mutants of IGPS designed to disrupt millisecond motions and allosteric coupling to identify regions that ...[more]