Dataset Information
Exercise induced upregulation of glutamate-cysteine ligase catalytic subunit and glutamate-cysteine ligase modifier subunit gene expression in Thoroughbred horses.
Ontology highlight
ABSTRACT: Objective
This study was performed to reveal the molecular structure and expression patterns of horse glutamate-cysteine ligase catalytic subunit (GCLC) and glutamate-cysteine ligase modifier subunit (GCLM) genes whose products form glutamate cysteine ligase, which were identified as differentially expressed genes in the previous study.Methods
We performed bioinformatics analyses, and gene expression assay with quantitative polymerase chain reaction (qPCR) for horse GCLC and GCLM genes in muscle and blood leukocytes of Thoroughbred horses.Results
Expression of GCLC showed the same pattern in both blood and muscle tissues after exercise. Expression of GCLC increased in the muscle and blood of Thoroughbreds, suggesting a tissue-specific regulatory mechanism for the expression of GCLC. In addition, expression of the GCLM gene increased after exercise in both the blood and muscle of Thoroughbreds.Conclusion
We established the expression patterns of GCLC and GCLM in the skeletal muscle and blood of Thoroughbred horses in response to exercise. Further study is now warranted to uncover the functional importance of these genes in exercise and recovery in racehorses.
SUBMITTER: Park JW
PROVIDER: S-EPMC5411833 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Publications
Mechanism of inactivation (desensitization) of acetylcholine receptor. Investigations by fast reaction techniques with membrane vesicles.
Biochemistry 19810601 12
Exposure of the acetylcholine receptor to acetylcholine, or its stable analogue carbamylcholine, inactivates (desensitizes) the receptor. Inactivation of receptor-controlled ion (86Rb+) flux in the presence of different concentrations of carbamylcholine (12.5 microM to 28 mM) was measured in the millisecond to minute time region, using a quench flow technique and membrane vesicles prepared from the electric organ of Electrophorus electricus. Three different kinetic measurements were made to esta ...[more]