Ontology highlight
ABSTRACT:
SUBMITTER: Burke TA
PROVIDER: S-EPMC5412564 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Burke Thomas A TA Harker Alyssa J AJ Dominguez Roberto R Kovar David R DR
The Journal of cell biology 20170331 5
VopL and VopF (VopL/F) are tandem WH2-domain actin assembly factors used by infectious <i>Vibrio</i> species to induce actin assembly in host cells. There is disagreement about the filament assembly mechanism of VopL/F, including whether they associate with the filament barbed or pointed end. Here, we used multicolor total internal reflection fluorescence microscopy to directly observe actin assembly with fluorescently labeled VopL/F. In actin monomer assembly reactions, VopL/F exclusively nucle ...[more]