Ontology highlight
ABSTRACT:
SUBMITTER: Winsor J
PROVIDER: S-EPMC5412568 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Winsor James J Hackney David D DD Lee Tina H TH
The Journal of cell biology 20170329 5
The homotypic fusion of endoplasmic reticulum membranes is catalyzed by the atlastin GTPase. The mechanism involves trans-dimerization between GTPase heads and a favorable crossover conformational shift, catalyzed by GTP hydrolysis, that converts the dimer from a "prefusion" to "postfusion" state. However, whether crossover formation actually energizes fusion remains unclear, as do the sequence of events surrounding it. Here, we made mutations in atlastin to selectively destabilize the crossover ...[more]