Ontology highlight
ABSTRACT:
SUBMITTER: Hennessy RC
PROVIDER: S-EPMC5412646 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Hennessy Rosanna C RC Stougaard Peter P Olsson Stefan S
Scientific reports 20170321 1
Here, we report the development of a microplate reader-based system for visualizing gene expression dynamics in living bacterial cells in response to a fungus in space and real-time. A bacterium expressing the red fluorescent protein mCherry fused to the promoter region of a regulator gene nunF indicating activation of an antifungal secondary metabolite gene cluster was used as a reporter system. Time-lapse image recordings of the reporter red signal and a green signal from fluorescent metabolit ...[more]