Ontology highlight
ABSTRACT:
SUBMITTER: Germini D
PROVIDER: S-EPMC5415314 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Germini Diego D Bou Saada Yara Y Tsfasman Tatiana T Osina Kristina K Robin Chloé C Lomov Nikolay N Rubtsov Mikhail M Sjakste Nikolajs N Lipinski Mar M Vassetzky Yegor Y
Molecular therapy. Methods & clinical development 20170308
Despite rapid progress, many problems and limitations persist and limit the applicability of gene-editing techniques. Making use of meganucleases, TALENs, or CRISPR/Cas9-based tools requires an initial step of pre-screening to determine the efficiency and specificity of the designed tools. This step remains time consuming and material consuming. Here we propose a simple, cheap, reliable, time-saving, and highly sensitive method to evaluate a given gene-editing tool based on its capacity to induc ...[more]