Ontology highlight
ABSTRACT:
SUBMITTER: Biel JT
PROVIDER: S-EPMC5415430 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Biel Justin T JT Thompson Michael C MC Cunningham Christian N CN Corn Jacob E JE Fraser James S JS
Structure (London, England : 1993) 20170413 5
Although protein design has been used to introduce new functions, designed variants generally only function as well as natural proteins after rounds of laboratory evolution. One possibility for this pattern is that designed mutants frequently sample nonfunctional conformations. To test this idea, we exploited advances in multiconformer modeling of room-temperature X-ray data collection on redesigned ubiquitin variants selected for increasing binding affinity to the deubiquitinase USP7. Initial c ...[more]