Ontology highlight
ABSTRACT:
SUBMITTER: Cain-Hom C
PROVIDER: S-EPMC5416772 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Cain-Hom Carol C Splinter Erik E van Min Max M Simonis Marieke M van de Heijning Monique M Martinez Maria M Asghari Vida V Cox J Colin JC Warming Søren S
Nucleic acids research 20170501 8
Cre/LoxP technology is widely used in the field of mouse genetics for spatial and/or temporal regulation of gene function. For Cre lines generated via pronuclear microinjection of a Cre transgene construct, the integration site is random and in most cases not known. Integration of a transgene can disrupt an endogenous gene, potentially interfering with interpretation of the phenotype. In addition, knowledge of where the transgene is integrated is important for planning of crosses between animals ...[more]