Ontology highlight
ABSTRACT:
SUBMITTER: Burnham DR
PROVIDER: S-EPMC5416776 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Burnham Daniel R DR Nijholt Bas B De Vlaminck Iwijn I Quan Jinhua J Yusufzai Timur T Dekker Cees C
Nucleic acids research 20170501 8
We investigate the mechanistic nature of the Snf2 family protein HARP, mutations of which are responsible for Schimke immuno-osseous dysplasia. Using a single-molecule magnetic tweezers assay, we construct RPA-stabilized DNA bubbles within torsionally constrained DNA to investigate the annealing action of HARP on a physiologically relevant substrate. We find that HARP closes RPA-stabilized bubbles in a slow reaction, taking on the order of tens of minutes for ∼600 bp of DNA to be re-annealed. Th ...[more]