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ABSTRACT:
SUBMITTER: Linden M
PROVIDER: S-EPMC5418599 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Lindén Martin M Ćurić Vladimir V Amselem Elias E Elf Johan J
Nature communications 20170503
Pointwise localization of individual fluorophores is a critical step in super-resolution localization microscopy and single particle tracking. Although the methods are limited by the localization errors of individual fluorophores, the pointwise localization precision has so far been estimated using theoretical best case approximations that disregard, for example, motion blur, defocus effects and variations in fluorescence intensity. Here, we show that pointwise localization precision can be accu ...[more]