Ontology highlight
ABSTRACT:
SUBMITTER: Jiang Y
PROVIDER: S-EPMC5418603 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Jiang Yu Y Qian Fenghui F Yang Junjie J Liu Yingmiao Y Dong Feng F Xu Chongmao C Sun Bingbing B Chen Biao B Xu Xiaoshu X Li Yan Y Wang Renxiao R Yang Sheng S
Nature communications 20170504
Corynebacterium glutamicum is an important industrial metabolite producer that is difficult to genetically engineer. Although the Streptococcus pyogenes (Sp) CRISPR-Cas9 system has been adapted for genome editing of multiple bacteria, it cannot be introduced into C. glutamicum. Here we report a Francisella novicida (Fn) CRISPR-Cpf1-based genome-editing method for C. glutamicum. CRISPR-Cpf1, combined with single-stranded DNA (ssDNA) recombineering, precisely introduces small changes into the bact ...[more]