Ontology highlight
ABSTRACT:
SUBMITTER: Schumacher D
PROVIDER: S-EPMC5418632 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Chemical science 20170320 5
The broad substrate tolerance of tubulin tyrosine ligase is the basic rationale behind its wide applicability for chemoenzymatic protein functionalization. In this context, we report that the wild-type enzyme enables ligation of various unnatural amino acids that are substantially bigger than and structurally unrelated to the natural substrate, tyrosine, without the need for extensive protein engineering. This unusual substrate flexibility is due to the fact that the enzyme's catalytic pocket fo ...[more]