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Redesigning the Q_A binding site of Photosystem II allows reduction of exogenous quinones.

ABSTRACT: Strategies to harness photosynthesis from living organisms to generate electrical power have long been considered, yet efficiency remains low. Here, we aimed to reroute photosynthetic electron flow in photosynthetic organisms without compromising their phototrophic properties. We show that 2,6-dimethyl-p-benzoquinone (DMBQ) can be used as an electron mediator to assess the efficiency of mutations designed to engineer a novel electron donation pathway downstream of the primary electron acceptor Q_A of Photosystem (PS) II in the green alga Chlamydomonas reinhardtii. Through the use of structural prediction studies and a screen of site-directed PSII mutants we show that modifying the environment of the Q_A site increases the reduction rate of DMBQ. Truncating the C-terminus of the PsbT subunit protruding in the stroma provides evidence that shortening the distance between Q_A and DMBQ leads to sustained electron transfer to DMBQ, as confirmed by chronoamperometry, consistent with a bypass of the natural Q_A°^- to Q_B pathway.

SUBMITTER: Fu HY

PROVIDER: S-EPMC5418674 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Redesigning the Q<sub>A</sub> binding site of Photosystem II allows reduction of exogenous quinones.

Fu Han-Yi HY Picot Daniel D Choquet Yves Y Longatte Guillaume G Sayegh Adnan A Delacotte Jérôme J Guille-Collignon Manon M Lemaître Frédéric F Rappaport Fabrice F Wollman Francis-André FA

Nature communications 20170503

Strategies to harness photosynthesis from living organisms to generate electrical power have long been considered, yet efficiency remains low. Here, we aimed to reroute photosynthetic electron flow in photosynthetic organisms without compromising their phototrophic properties. We show that 2,6-dimethyl-p-benzoquinone (DMBQ) can be used as an electron mediator to assess the efficiency of mutations designed to engineer a novel electron donation pathway downstream of the primary electron acceptor Q ...[more]

PMID: 28466860

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Project description:Calcium enhances turfgrass response to salt stress. However, little is known about PSII photochemical changes when exogenous calcium was applied in salinity-stressed turfgrass. Here, we probe into the rearrangements of PSII electron transport and endogenous ion accumulation in tall fescue (Festuca arundinacea Schreber) treated with exogenous calcium under salt stress. Three-month-old seedlings of genotype "TF133" were subjected to the control (CK), salinity (S), salinity + calcium nitrate (SC), and salinity + ethylene glycol tetraacetic acid (SE). Calcium nitrate and ethylene glycol tetraacetic acid was used as exogenous calcium donor and calcium chelating agent respectively. At the end of a 5-day duration treatment, samples in SC regime had better photochemistry performance on several parameters than salinity only. Such as the Area (equal to the plastoquinone pool size), N (number of [Formula: see text] redox turnovers until Fm is reached), ?E0, or ?Ro (Efficiencdy/probability with which a PSII trapped electron is transferred from QA to QB or PSI acceptors), ABS/RC (Absorbed photon flux per RC). All the above suggested that calcium enhanced the electron transfer of PSII (especially beyond [Formula: see text]) and prevented reaction centers from inactivation in salt-stressed tall fescue. Furthermore, both grass shoot and root tissues generally accumulated more C, N, Ca2+, and K+ in the SC regime than S regime. Interrelated analysis indicated that ?E0, ?Ro, ABS/RC, C, and N content in shoots was highly correlated to each other and significantly positively related to Ca2+ and K+ content in roots. Besides, high salt increased ATP6E and CAMK2 transcription level in shoot at 1 and 5 day, respectively while exogenous calcium relieved it. In root, CAMK2 level was reduced by Salinity at 5 day and exogenous calcium recovered it. These observations involved in electron transport capacity and ion accumulation assist in understanding better the protective role of exogenous calcium in tall fescue under salt stress.

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Redesigning the Q_A binding site of Photosystem II allows reduction of exogenous quinones.

Publications

Redesigning the Q<sub>A</sub> binding site of Photosystem II allows reduction of exogenous quinones.

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Dataset Information

Redesigning the QA binding site of Photosystem II allows reduction of exogenous quinones.

Publications

Redesigning the Q<sub>A</sub> binding site of Photosystem II allows reduction of exogenous quinones.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

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Redesigning the Q_A binding site of Photosystem II allows reduction of exogenous quinones.