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Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay.


ABSTRACT: Escherichia coli O157:H7 is known to cause serious diseases including hemorrhagic colitis and hemolytic uremic syndrome. A gold nanoparticle lateral flow immunoassay (Au-LFIA) was used to detect Escherichia coli O157:H7 in ground pork samples. False-positive results were detected using Au-LFIA; a Citrobacterfreundii strain was isolated from the ground pork samples and identified by using CHROmagarTM plates, API 20E, and 16S RNA sequencing. Since C.freundii showed cross-reactivity with E. coli O157:H7 when Au-LFIA test strips were used, a novel method combining modified enrichment with a lateral flow immunoassay for accurate and convenient detection of E. coli O157:H7 in ground pork was developed in this study to minimize these false positives. MacConkey broth was optimized for E. coli O157:H7 enrichment and C.freundii inhibition by the addition of 5 mg/L potassium tellurite and 0.10 mg/L cefixime. Using the proposed modified enrichment procedure, the false-positive rate of ground pork samples spiked with 100 CFU/g C.freundii decreased to 5%.

SUBMITTER: Cheng S 

PROVIDER: S-EPMC5421713 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay.

Cheng Song S   Chen Ming-Hui MH   Zhang Gang-Gang GG   Yu Zhi-Biao ZB   Liu Dao-Feng DF   Xiong Yong-Hua YH   Wei Hua H   Lai Wei-Hua WH  

Sensors (Basel, Switzerland) 20170402 4


<i>Escherichia coli</i> O157:H7 is known to cause serious diseases including hemorrhagic colitis and hemolytic uremic syndrome. A gold nanoparticle lateral flow immunoassay (Au-LFIA) was used to detect <i>Escherichia coli</i> O157:H7 in ground pork samples. False-positive results were detected using Au-LFIA; a <i>Citrobacter</i><i>freundii</i> strain was isolated from the ground pork samples and identified by using CHROmagar<sup>TM</sup> plates, API 20E, and 16S RNA sequencing. Since <i>C.</i><i  ...[more]

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