Ontology highlight
ABSTRACT:
SUBMITTER: Wong JJ
PROVIDER: S-EPMC5424149 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Wong Justin J-L JJ Gao Dadi D Nguyen Trung V TV Kwok Chau-To CT van Geldermalsen Michelle M Middleton Rob R Pinello Natalia N Thoeng Annora A Nagarajah Rajini R Holst Jeff J Ritchie William W Rasko John E J JEJ
Nature communications 20170508
While intron retention (IR) is considered a widely conserved and distinct mechanism of gene expression control, its regulation is poorly understood. Here we show that DNA methylation directly regulates IR. We also find reduced occupancy of MeCP2 near the splice junctions of retained introns, mirroring the reduced DNA methylation at these sites. Accordingly, MeCP2 depletion in tissues and cells enhances IR. By analysing the MeCP2 interactome using mass spectrometry and RNA co-precipitation, we de ...[more]