Ontology highlight
ABSTRACT:
SUBMITTER: Sim YJ
PROVIDER: S-EPMC5425728 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Sim Ye-Ji YJ Kim Min-Seong MS Nayfeh Abeer A Yun Ye-Jin YJ Kim Su-Jin SJ Park Kyung-Tae KT Kim Chang-Hoon CH Kim Kye-Seong KS
Stem cell reports 20170427 5
Mouse embryonic stem cells (ESCs) are maintained in serum with leukemia inhibitory factor (LIF) to maintain self-renewal and pluripotency. Recently, a 2i culture method was reported using a combination of MEK inhibition (MEKi) and GSK3 inhibition (GSK3i) with LIF to maintain ESCs in a naive ground state. How 2i maintains a ground state of ESCs remains elusive. Here we show that MEKi and GSK3i maintain the ESC ground state by downregulating global DNA methylation through two distinct mechanisms. ...[more]