RNA Polymerase III Subunit POLR3G Regulates Specific Subsets of PolyA⁺ and SmallRNA Transcriptomes and Splicing in Human Pluripotent Stem Cells.

ABSTRACT: POLR3G is expressed at high levels in human pluripotent stem cells (hPSCs) and is required for maintenance of stem cell state through mechanisms not known in detail. To explore how POLR3G regulates stem cell state, we carried out deep-sequencing analysis of polyA⁺ and smallRNA transcriptomes present in hPSCs and regulated in POLR3G-dependent manner. Our data reveal that POLR3G regulates a specific subset of the hPSC transcriptome, including multiple transcript types, such as protein-coding genes, long intervening non-coding RNAs, microRNAs and small nucleolar RNAs, and affects RNA splicing. The primary function of POLR3G is in the maintenance rather than repression of transcription. The majority of POLR3G polyA⁺ transcriptome is regulated during differentiation, and t

SUBMITTER: Lund RJ 

PROVIDER: S-EPMC5425787 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature