Mincle-Mediated Neutrophil Extracellular Trap Formation by Regulation of Autophagy.

ABSTRACT:

Background

Neutrophil extracellular traps (NETs) constitute antimicrobial function of neutrophils but have also been linked to perpetuation of inflammation. Despite this evident physiological relevance, mechanistic understanding of NET formation is poor. In this study, we examined the mechanism by which Mincle, a C-type lectin receptor, regulates NET formation.

Methods

NET formation, reactive oxygen species, autophagy activation and intracellular signaling pathways were analyzed in Mincle-sufficient and -deficient neutrophils stimulated in vitro with various stimuli and in vivo during Klebsiella infection.

Results

We found that Mincle mediates NET formation in response to several activation stimuli in vitro and in vivo during pneumoseptic infection with Klebsiella pne

SUBMITTER: Sharma A 

PROVIDER: S-EPMC5426377 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature