Isolation, cloning, and characterization of a cuticle collagen gene, Mi-col-5, in Meloidogyne incognita.

ABSTRACT: Cuticle collagens form a major part of the nematode cuticle and are responsible for maintaining the overall shape of the animal and its protection from the external environment. Although substantial research on cuticle collagen genes has been carried out in Caenorhabditis elegans, their isolation and characterization in plant parasitic nematodes have been limited to a few genes only. In this study, a cuticle collagen gene, Mi-col-5, was isolated from root-knot nematode, Meloidogyne incognita. A partial segment of 402 bp was first cloned and analyzed on Gbrowse followed by subsequent cloning of the 1047 bp long full cDNA specifying the open reading frame. The deduced amino acid sequence showed 92% sequence identity with that of Mj-col-5. However, a transmembrane helix was predicted in Mi-co

SUBMITTER: Banerjee S 

PROVIDER: S-EPMC5428120 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature