Hyperosmolarity impedes the cross-priming competence of dendritic cells in a TRIF-dependent manner.

ABSTRACT: Tissue osmolarity varies among different organs and can be considerably increased under pathologic conditions. Hyperosmolarity has been associated with altered stimulatory properties of immune cells, especially macrophages and dendritic cells. We have recently reported that dendritic cells upon exposure to hypertonic stimuli shift their profile towards a macrophage-M2-like phenotype, resulting in attenuated local alloreactivity during acute kidney graft rejection. Here, we examined how hyperosmotic microenvironment affects the cross-priming capacity of dendritic cells. Using ovalbumin as model antigen, we showed that exposure of dendritic cells to hyperosmolarity strongly inhibits activation of antigen-specific T cells despite enhancement of antigen uptake, processing and presentation. We

SUBMITTER: Popovic ZV 

PROVIDER: S-EPMC5428499 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature