Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion.
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ABSTRACT:
SUBMITTER: Nekrasov V
PROVIDER: S-EPMC5428673 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion.
Nekrasov Vladimir V Wang Congmao C Win Joe J Lanz Christa C Weigel Detlef D Kamoun Sophien S
Scientific reports 20170328 1
Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/C ...[more]
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