Dataset Information

Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats.

ABSTRACT: This study was performed to investigate the initial colonization of metabolically active methanogens and subsequent changes in four fractions: the rumen solid-phase (RS), liquid-phase (RL), protozoa-associated (RP), and epithelium-associated (RE) from 1 to 60?d after birth, and manipulate methanogen community by early weaning on 40?d and supplementing rhubarb from 40 to 60?d in black goats. The RNA-based real-time quantitative PCR and 16S rRNA amplicon sequencing were employed to indicate the metabolically active methanogens. Results showed that active methanogens colonized in RL and RE on 1?d after birth. RP and RE contained the highest and lowest density of methanogens, respectively. Methanobrevibacter, Candidatus Methanomethylophilus, and Methanosphaera were the top three genera. The methanogen communities before weaning differed from those post weaning and the structure of the methanogen community in RE was distinct from those in the other three fractions. The discrepancies in the distribution of methanogens across four fractions, and various fluctuations in abundances among four fractions according to age were observed. The addition of rhubarb significantly (P?

SUBMITTER: Wang Z

PROVIDER: S-EPMC5428682 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats.

Wang Zuo Z Elekwachi Chijioke O CO Jiao Jinzhen J Wang Min M Tang Shaoxun S Zhou Chuanshe C Tan Zhiliang Z Forster Robert J RJ

Scientific reports 20170324 1

This study was performed to investigate the initial colonization of metabolically active methanogens and subsequent changes in four fractions: the rumen solid-phase (RS), liquid-phase (RL), protozoa-associated (RP), and epithelium-associated (RE) from 1 to 60 d after birth, and manipulate methanogen community by early weaning on 40 d and supplementing rhubarb from 40 to 60 d in black goats. The RNA-based real-time quantitative PCR and 16S rRNA amplicon sequencing were employed to indicate the me ...[more]

PMID: 28341835

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Dataset Information

Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats.

Publications

Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats.

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