Dataset Information

Response of chickpea (Cicer arietinum L.) to terminal drought: leaf stomatal conductance, pod abscisic acid concentration, and seed set.

ABSTRACT: Flower and pod production and seed set of chickpea (Cicer arietinum L.) are sensitive to drought stress. A 2-fold range in seed yield was found among a large number of chickpea genotypes grown at three dryland field sites in south-western Australia. Leaf water potential, photosynthetic characteristics, and reproductive development of two chickpea genotypes with contrasting yields in the field were compared when subjected to terminal drought in 106kg containers of soil in a glasshouse. The terminal drought imposed from early podding reduced biomass, reproductive growth, harvest index, and seed yield of both genotypes. Terminal drought at least doubled the percentage of flower abortion, pod abscission, and number of empty pods. Pollen viability and germination decreased when the fraction of transpirable soil water (FTSW) decreased below 0.18 (82% of the plant-available soil water had been transpired); however, at least one pollen tube in each flower reached the ovary. The young pods which developed from flowers produced when the FTSW was 0.50 had viable embryos, but contained higher abscisic acid (ABA) concentrations than those of the well-watered plants; all pods ultimately aborted in the drought treatment. Cessation of seed set at the same soil water content at which stomata began to close and ABA increased strongly suggested a role for ABA signalling in the failure to set seed either directly through abscission of developing pods or seeds or indirectly through the reduction of photosynthesis and assimilate supply to the seeds.

SUBMITTER: Pang J

PROVIDER: S-EPMC5429003 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Response of chickpea (Cicer arietinum L.) to terminal drought: leaf stomatal conductance, pod abscisic acid concentration, and seed set.

Pang Jiayin J Turner Neil C NC Khan Tanveer T Du Yan-Lei YL Xiong Jun-Lan JL Colmer Timothy D TD Devilla Rosangela R Stefanova Katia K Siddique Kadambot H M KHM

Journal of experimental botany 20170401 8

Flower and pod production and seed set of chickpea (Cicer arietinum L.) are sensitive to drought stress. A 2-fold range in seed yield was found among a large number of chickpea genotypes grown at three dryland field sites in south-western Australia. Leaf water potential, photosynthetic characteristics, and reproductive development of two chickpea genotypes with contrasting yields in the field were compared when subjected to terminal drought in 106kg containers of soil in a glasshouse. The termin ...[more]

PMID: 27099375

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Project description:Elucidation of the genetic basis of drought tolerance is vital for genomics-assisted breeding of drought tolerant crop varieties. Here, we used genotyping-by-sequencing (GBS) to identify single nucleotide polymorphisms (SNPs) in recombinant inbred lines (RILs) derived from a cross between a drought tolerant chickpea variety, Pusa 362 and a drought sensitive variety, SBD 377. The GBS identified a total of 35,502 SNPs and subsequent filtering of these resulted in 3237 high-quality SNPs included in the eight linkage groups. Fifty-one percent of these SNPs were located in the genic regions distributed throughout the genome. The high density linkage map has total map length of 1069 cm with an average marker interval of 0.33 cm. The linkage map was used to identify 9 robust and consistent QTLs for four drought related traits viz. membrane stability index, relative water content, seed weight and yield under drought, with percent variance explained within the range of 6.29%-90.68% and LOD scores of 2.64 to 6.38, which were located on five of the eight linkage groups. A genomic region on LG 7 harbors quantitative trait loci (QTLs) explaining > 90% phenotypic variance for membrane stability index, and > 10% PVE for yield. This study also provides the first report of major QTLs for physiological traits such as membrane stability index and relative water content for drought stress in chickpea. A total of 369 putative candidate genes were identified in the 6.6 Mb genomic region spanning these QTLs. In-silico expression profiling based on the available transcriptome data revealed that 326 of these genes were differentially expressed under drought stress. KEGG analysis resulted in reduction of candidate genes from 369 to 99, revealing enrichment in various signaling pathways. Haplotype analysis confirmed 5 QTLs among the initially identified 9 QTLs. Two QTLs, qRWC1.1 and qYLD7.1, were chosen based on high SNP density. Candidate gene-based analysis revealed distinct haplotypes in qYLD7.1 associated with significant phenotypic differences, potentially linked to pathways for secondary metabolite biosynthesis. These identified candidate genes bolster defenses through flavonoids and phenylalanine-derived compounds, aiding UV protection, pathogen resistance, and plant structure.The study provides novel genomic regions and candidate genes which can be utilized in genomics-assisted breeding of superior drought tolerant chickpea cultivars.

Project description:Chickpea is the second most important legume crop largely grown under semi-arid tropics where terminal drought is one of the major constraints for its productivity. A trait-based selection had been considered more beneficial in drought tolerance breeding to overcome the environmental influence on drought yields. Large number of traits had been suggested in literature, with less indication on their importance and priority, for use in such breeding programs resulting in poor utilization of critical traits in drought tolerance breeding. To identify the most critical traits that contribute to grain yield under drought, 12 chickpea genotypes, with well-defined drought response, were field evaluated by sampling at regular intervals during the cropping period. Large range of variation was observed for shoot biomass productivity, specific leaf area (SLA) and leaf area index (LAI) at different days after sowings (DAS), canopy temperature depression (CTD) at mid-reproductive stages, growth duration and both morphological and analytical yield components. Grain yield under drought was closely associated with the rate of partitioning (p), crop growth rate (C), CTD, phenology, LAI at mid-pod fill stage, pod number m-2 at maturity, shoot biomass at reproductive growth stages and SLA at physiological maturity. The shoot trait(s) were prioritized based on their significance and contribution to drought tolerance. The trait(s) that conferred tolerance varied across genotypes. The order of traits/plant functions identified as important and critical for the drought tolerance were p, C, CTD, growth duration and other related traits. Relatively less important traits were LAI, SLA at the mid reproductive stage and pod number per unit area at maturity. The traits Dr, seeds pod-1 and 100-seed weight were found to be least important. Breeding for the best combination of p and C with the right phenology was proposed to be the best selection strategy to enhance terminal drought tolerance in chickpea.

Dataset Information

Response of chickpea (Cicer arietinum L.) to terminal drought: leaf stomatal conductance, pod abscisic acid concentration, and seed set.

Publications

Response of chickpea (Cicer arietinum L.) to terminal drought: leaf stomatal conductance, pod abscisic acid concentration, and seed set.

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