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A benign alternative process for efficient separation of pure commercially important flavonoid nutraceuticals from edible plants.

ABSTRACT: The present study signifies the development of a unique, optimized procedure for both selective determination and separation of different flavonoid nutraceuticals from edible plants. Totally ten different flavonoids were determined (HPLC-DAD) and isolated from five different plants using the developed process with a remarkable purity of 91-98% and recovery of 88-95%. Box-Behnken experimental design model yielded a optimized amount of 40.36 mg/g of AI extract (Pinostrobin) and 28.95 mg/g of AI extract (Baicalein) with a high correlation coefficient (0.98-0.99), indicating a good fit between the second order regression model and the experimental observations. The final purity of compounds through optimized process is 97.1% (Pinostrobin) and 93.5% (Baicalein) respectively. The optimized yields depicted a total recovery of 92% for pinostrobin, and 89% for Baicalein respectively. Thus, the developed process worked as a potential alternative which when statistically optimized results in a remarkable recovery of flavonoids from various plants. Being an environmentally friendly protocol the process could be useful in industrial separation of commercially important flavonoids widely applied in food industry.

SUBMITTER: Subramaniam S

PROVIDER: S-EPMC5430184 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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A benign alternative process for efficient separation of pure commercially important flavonoid nutraceuticals from edible plants.

Subramaniam Shankar S Raju Ravikumar R Sivasubramanian Aravind A

Journal of food science and technology 20170322 6

The present study signifies the development of a unique, optimized procedure for both selective determination and separation of different flavonoid nutraceuticals from edible plants. Totally ten different flavonoids were determined (HPLC-DAD) and isolated from five different plants using the developed process with a remarkable purity of 91-98% and recovery of 88-95%. Box-Behnken experimental design model yielded a optimized amount of 40.36 mg/g of AI extract (Pinostrobin) and 28.95 mg/g of AI ex ...[more]

PMID: 28559611

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Project description:Freshness is considered one of the most important parameters to judge the quality of most fish products. In the current study, the seasonality effect on the freshness profile of different economic fish species was evaluated for the first time, using three different approaches (sensory: Quality Index Method (QIM) and European (EC) Scheme; physical: Torrymeter (TRM) values; and microbiological analyses: Total Viable Counts (TVC) and degradative bacteria). Over a year, individuals of farmed fish Sparus aurata and Dicentrarchus labrax, as well as the wild fish Trachurus trachurus, Scomber colias, and Sardina pilchardus, were sampled seasonally for the evaluation of their freshness profile over 10 days on ice. In general, data showed an increase in QIM values, a decline in TRM, and an increase of spoilage bacteria throughout the storage time, revealing a clear temporal degradation of the quality of the fish. Additionally, some signs of seasonality effect could only be observed for some species. For example, the seabass D. labrax showed lower numbers of degradative bacteria in winter than in the other seasons, suggesting a high potential to be marketed in a fresher condition, especially during that season. On the other hand, S. colias showed higher freshness scores (i.e., higher TRM values in spring and autumn and lower numbers of bacteria in summer) from spring to autumn. However, from the five studied species, S. colias presented the lowest freshness values, indicating a higher fragility of this species. This information is extremely relevant for consumers and retailers that want to invest in higher quality products, as they would thus be able to choose certain species in detriment of others. Additionally, obtained data showed that farmed species reached day 10 of storage time with lower values of QIM and microbial counts (cfu), as well as higher values of TRM, in relation to wild species. These results reinforce the idea that farmed fish can, under proper conditions, present high quality/freshness profile.

Dataset Information

A benign alternative process for efficient separation of pure commercially important flavonoid nutraceuticals from edible plants.

Publications

A benign alternative process for efficient separation of pure commercially important flavonoid nutraceuticals from edible plants.

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