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Identification of food preservative, stress relief compounds by GC-MS and HR-LC/Q-TOF/MS; evaluation of antioxidant activity of Acalypha indica leaves methanolic extract (in vitro) and polyphenolic fraction (in vivo).

ABSTRACT: The present paper has been designed to evaluate phytochemical profile, in vitro free radical scavenging activity, cytotoxicity of methanolic extract and in vivo antioxidant activity of polyphenolic fraction of Acalypha indica leaves. Methanolic extract of A. indica leaves (MEAIL) contained rich amount of phenols, flavonoids and saponins. The GC-MS analysis of extract revealed 13 compounds, whereas HR-LC/Q-TOF/MS showed 87, and all were coincided with functional groups identified by FTIR. The extract showed good scavenging activity on DPPH, H₂O₂, hydroxyl radicals and metal ions. The Polyphenolic fraction induced the antioxidant enzymes in Diabetic rats. The extract also potentially showed cytotoxic (LC₅₀: 140.02 µg/mL) activity against brine shrimp. Based on these analytical results, in vitro and in vivo experiments, it was concluded that the MEAIL has encompassed rich amount of polyphenols (antioxidants) and cytotoxic compounds for their respective activities. Polyphenolic fraction has the induction capacity to elevate cellular antioxidant enzymes in diabetic animals.

SUBMITTER: Ravi S

PROVIDER: S-EPMC5430191 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Identification of food preservative, stress relief compounds by GC-MS and HR-LC/Q-TOF/MS; evaluation of antioxidant activity of Acalypha indica leaves methanolic extract (in vitro) and polyphenolic fraction (in vivo).

Ravi Sahukari S Shanmugam Bhasha B Subbaiah Ganjikunta Venkata GV Prasad Singamala Hari SH Reddy Kesireddy Sathyavelu KS

Journal of food science and technology 20170403 6

The present paper has been designed to evaluate phytochemical profile, in vitro free radical scavenging activity, cytotoxicity of methanolic extract and in vivo antioxidant activity of polyphenolic fraction of Acalypha indica leaves. Methanolic extract of A. indica leaves (MEAIL) contained rich amount of phenols, flavonoids and saponins. The GC-MS analysis of extract revealed 13 compounds, whereas HR-LC/Q-TOF/MS showed 87, and all were coincided with functional groups identified by ...[more]

PMID: 28559618

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Project description:(1) Background: The ability to determine the age of ginseng is very important because the price of ginseng depends on the cultivation period. Since morphological observation is subjective, a new scientific and systematic method for determining the age of ginseng is required. (2) Methods: Three techniques were used for a metabolomics approach. High-resolution magic-angle-spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy was used to analyze powdered ginseng samples without extraction. Ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) and gas chromatography quadrupole time-of-fight mass spectrometry (GC-TOF/MS) were used to analyze the extracts of 4-, 5-, and 6-year-old ginseng. (3) Results: A metabolomics approach has the potential to discriminate the age of ginseng. Among the primary metabolites detected from NMR spectroscopy, the levels of fumarate and choline showed moderate prediction with an area under the curve (AUC) value of more than 0.7. As a result of UPLC-QTOF/MS-based profiling, 61 metabolites referring to the VIP (variable importance in the projection) score contributed to discriminating the age of ginseng. The results of GC×GC-TOF/MS showed clear discrimination of 4-, 5-, and 6-year-old ginseng using orthogonal partial least-squares discriminant analysis (OPLS-DA) to 100% of the discrimination rate. The results of receiver operating characteristic (ROC) analysis, 16 metabolites between 4- and 5-year-old ginseng, and 18 metabolites between 5- and 6-year-old ginseng contributed to age discrimination in all regions. (4) Conclusions: These results showed that metabolic profiling and multivariate statistical analyses can distinguish the age of ginseng. Especially, it is meaningful that ginseng samples from different areas had the same metabolites for age discrimination. In future studies, it will be necessary to identify the unknown variables and to collaboratively study with other fields the biochemistry of aging in ginseng.

Dataset Information

Identification of food preservative, stress relief compounds by GC-MS and HR-LC/Q-TOF/MS; evaluation of antioxidant activity of Acalypha indica leaves methanolic extract (in vitro) and polyphenolic fraction (in vivo).

Publications

Identification of food preservative, stress relief compounds by GC-MS and HR-LC/Q-TOF/MS; evaluation of antioxidant activity of <i>Acalypha indica</i> leaves methanolic extract (in vitro) and polyphenolic fraction (in vivo).

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