Ontology highlight
ABSTRACT:
SUBMITTER: Torres M
PROVIDER: S-EPMC5430456 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Torres Marta M Uroz Stéphane S Salto Rafael R Fauchery Laure L Quesada Emilia E Llamas Inmaculada I
Scientific reports 20170419 1
The screening of a metagenomic library of 250,000 clones generated from a hypersaline soil (Spain) allowed us to identify a single positive clone which confers the ability to degrade N-acyl homoserine lactones (AHLs). The sequencing of the fosmid revealed a 42,318 bp environmental insert characterized by 46 ORFs. The subcloning of these ORFs demonstrated that a single gene (hqiA) allowed AHL degradation. Enzymatic analysis using purified HqiA and HPLC/MS revealed that this protein has lactonase ...[more]