Ontology highlight
ABSTRACT:
SUBMITTER: Rogov VV
PROVIDER: S-EPMC5430633 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Rogov Vladimir V VV Suzuki Hironori H Marinković Mija M Lang Verena V Kato Ryuichi R Kawasaki Masato M Buljubašić Maja M Šprung Matilda M Rogova Natalia N Wakatsuki Soichi S Hamacher-Brady Anne A Dötsch Volker V Dikic Ivan I Brady Nathan R NR Novak Ivana I
Scientific reports 20170425 1
The mitophagy receptor Nix interacts with LC3/GABARAP proteins, targeting mitochondria into autophagosomes for degradation. Here we present evidence for phosphorylation-driven regulation of the Nix:LC3B interaction. Isothermal titration calorimetry and NMR indicate a ~100 fold enhanced affinity of the serine 34/35-phosphorylated Nix LC3-interacting region (LIR) to LC3B and formation of a very rigid complex compared to the non-phosphorylated sequence. Moreover, the crystal structure of LC3B in co ...[more]