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High throughput single cell counting in droplet-based microfluidics.

ABSTRACT: Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adherent and non-adherent human cells as well as bacteria. Our microfluidic hemocytometer provides statistically relevant data on large populations of cells at a high-throughput, used to characterize cell encapsulation and cell viability during incubation in droplets.

SUBMITTER: Lu H

PROVIDER: S-EPMC5431057 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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High throughput single cell counting in droplet-based microfluidics.

Lu Heng H Caen Ouriel O Vrignon Jeremy J Zonta Eleonora E El Harrak Zakaria Z Nizard Philippe P Baret Jean-Christophe JC Taly Valérie V

Scientific reports 20170502 1

Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adherent and non-adherent human cells as well as bacteria. Our microfluidic hemocytometer provides statistically relevant data on large populations of cells at a high-throughput, used to characterize cell encapsu ...[more]

PMID: 28465615

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