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A simple and cost-effective setup for super-resolution localization microscopy.

ABSTRACT: Single molecule localization microscopy (SMLM) has become a powerful imaging tool for biomedical research, but it is mostly available in imaging facilities and a small number of laboratories due to its high cost. Here, we evaluate the possibility of replacing high-cost components on standard SMLM with appropriate low-cost alternatives and build a simple but high-performance super-resolution SMLM setup. Through numerical simulation and biological experiments, we demonstrate that our low-cost SMLM setup can yield similar localization precision and spatial resolution compared to the standard SMLM equipped with state-of-the-art components, but at a small fraction of their cost. Our low-cost SMLM setup can potentially serve as a routine laboratory microscope with high-performance super-resolution imaging capability.

SUBMITTER: Ma H

PROVIDER: S-EPMC5431525 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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A simple and cost-effective setup for super-resolution localization microscopy.

Ma Hongqiang H Fu Rao R Xu Jianquan J Liu Yang Y

Scientific reports 20170508 1

Single molecule localization microscopy (SMLM) has become a powerful imaging tool for biomedical research, but it is mostly available in imaging facilities and a small number of laboratories due to its high cost. Here, we evaluate the possibility of replacing high-cost components on standard SMLM with appropriate low-cost alternatives and build a simple but high-performance super-resolution SMLM setup. Through numerical simulation and biological experiments, we demonstrate that our low-cost SMLM ...[more]

PMID: 28484239

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