Development of a S-adenosylmethionine analog that intrudes the RNA-cap binding site of Zika methyltransferase.
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ABSTRACT:
SUBMITTER: Jain R
PROVIDER: S-EPMC5431627 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Publications
Development of a S-adenosylmethionine analog that intrudes the RNA-cap binding site of Zika methyltransferase.
Jain Rinku R Butler Kyle V KV Coloma Javier J Jin Jian J Aggarwal Aneel K AK
Scientific reports 20170509 1
The Zika virus (ZIKV) has emerged as a major health hazard. We present here a high resolution structure (1.55 Å) of ZIKV NS5 methyltransferase bound to a novel S-adenosylmethionine (SAM) analog in which a 4-fluorophenyl moiety substitutes for the methyl group. We show that the 4-fluorophenyl moiety extends into a portion of the RNA binding tunnel that typically contains the adenosine 2'OH of the RNA-cap moiety. Together, the new SAM analog and the high-resolution crystal structure are a step tow ...[more]
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