Genotyping-by-sequencing of three mapping populations for identification of candidate genomic regions for resistance to sterility mosaic disease in pigeonpea.

ABSTRACT: Sterility mosaic disease (SMD) is one of the serious production constraints that may lead to complete yield loss in pigeonpea. Three mapping populations including two recombinant inbred lines and one F₂, were used for phenotyping for SMD resistance at two locations in three different years. Genotyping-by-sequencing approach was used for simultaneous identification and genotyping of SNPs on above mentioned populations. In total, 212,464, 89,699 and 64,798 SNPs were identified in ICPL 20096?×?ICPL 332 (PRIL_B), ICPL 20097?×?ICP 8863 (PRIL_C) and ICP 8863?×?ICPL 87119 (F₂) respectively. By using high-quality SNPs, genetic maps were developed for PRIL_B (1,101 SNPs; 921.21?cM), PRIL_C (484 SNPs; 798.25?cM) and F₂ (996 SNPs; 1,597.30?cM) populations. The average inter marker distance on these maps varied from 0.84?cM to 1.65?cM, which was lowest in all genetic mapping studies in pigeonpea. Composite interval mapping based QTL analysis identified a total of 10?QTLs including three major QTLs across the three populations. The phenotypic variance of the identified QTLs ranged from 3.6 to 34.3%. One candidate genomic region identified on CcLG11 seems to be promising QTL for molecular breeding in developing superior lines with enhanced resistance to SMD.

SUBMITTER: Saxena RK 

PROVIDER: S-EPMC5431754 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature