Characterization of LhSorP5CS, a gene catalyzing proline synthesis in Oriental hybrid lily Sorbonne: molecular modelling and expression analysis.

ABSTRACT:

Background

Abiotic stresses negatively affect plant growth and flower production. In plants, P5CS proteins are key enzymes that catalyzed the rate-limiting steps of proline synthesis, and proline is a well-known osmoprotectant that is closely related to abiotic stress tolerance. However, information about the P5CS genes, their effects on proline accumulation, and their role in abiotic stress tolerance in Lilium is still lacking.

Results

We isolated and characterized a novel gene (LhSorP5CS) from Oriental hybrid lily cultivar Sorbonne. Phylogenetic analysis indicated that LhSorP5CS is a member of the P5CS family. The three-dimensional structure of LhSorP5CS predicted by homology modeling showed high similarity to its correspondant human P5CS template. Further gene expression

SUBMITTER: Wang L 

PROVIDER: S-EPMC5432930 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature