Dataset Information

Isoflavone Malonyltransferases GmIMaT1 and GmIMaT3 Differently Modify Isoflavone Glucosides in Soybean (Glycine max) under Various Stresses.

ABSTRACT: Malonylated isoflavones are the major forms of isoflavonoids in soybean plants, the genes responsible for their biosyntheses are not well understood, nor their physiological functions. Here we report a new benzylalcohol O-acetyltransferase, anthocyanin O-hydroxycinnamoyltransferase, anthranilate N-hydroxycinnamoyl/benzoyltransferase, deacetylvindoline 4-O-acetyltransferase (BAHD) family isoflavone glucoside malonyltransferase GmIMaT1, and GmIMaT3, which is allelic to the previously characterized GmMT7 and GmIF7MaT. Biochemical studies showed that recombinant GmIMaT1 and GmIMaT3 enzymes used malonyl-CoA and several isoflavone 7-O-glucosides as substrates. The Km values of GmIMaT1 for glycitin, genistin, and daidzin were 13.11, 23.04, and 36.28 ?M, respectively, while these of GmIMaT3 were 12.94, 26.67, and 30.12 ?M, respectively. Transgenic hairy roots overexpressing both GmIMaTs had increased levels of malonyldaidzin and malonylgenistin, and contents of daidzin and glycitin increased only in GmIMaT1-overexpression lines. The increased daidzein and genistein contents were detected only in GmIMaT3-overexpression lines. Knockdown of GmIMaT1 and GmIMaT3 reduced malonyldaidzin and malonylgenistin contents, and affected other isoflavonoids differently. GmIMaT1 is primarily localized to the endoplasmic reticulum while GmIMaT3 is primarily in the cytosol. By examining their transcript changes corresponding to the altered isoflavone metabolic profiles under various environmental and hormonal stresses, we probed the possible functions of GmIMaTs. Two GmIMaTs displayed distinct tissue expression patterns and respond differently to various factors in modifying isoflavone 7-O-glucosides under various stresses.

SUBMITTER: Ahmad MZ

PROVIDER: S-EPMC5433297 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Isoflavone Malonyltransferases GmIMaT1 and GmIMaT3 Differently Modify Isoflavone Glucosides in Soybean (Glycine max) under Various Stresses.

Ahmad Muhammad Z MZ Li Penghui P Wang Junjie J Rehman Naveed Ur NU Zhao Jian J

Frontiers in plant science 20170516

Malonylated isoflavones are the major forms of isoflavonoids in soybean plants, the genes responsible for their biosyntheses are not well understood, nor their physiological functions. Here we report a new benzylalcohol O-acetyltransferase, anthocyanin O-hydroxycinnamoyltransferase, anthranilate N-hydroxycinnamoyl/benzoyltransferase, deacetylvindoline 4-O-acetyltransferase (BAHD) family isoflavone glucoside malonyltransferase GmIMaT1, and GmIMaT3, which is allelic to the pre ...[more]

PMID: 28559900

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Project description:BackgroundSoybean is a valuable source of edible protein and oil, as well as secondary metabolites that can be used in food products, cosmetics, and medicines. However, because soybean isoflavone content is a quantitative trait influenced by polygenes and environmental interactions, its genetic basis remains unclear.ResultsThis study was conducted to identify causal quantitative trait loci (QTLs) associated with soybean isoflavone contents. A mutant-based F2 population (190 individuals) was created by crossing the Korean cultivar Hwanggeum with low isoflavone contents (1,558 µg g-1) and the soybean mutant DB-088 with high isoflavone contents (6,393 µg g-1). A linkage map (3,049 cM) with an average chromosome length of 152 cM was constructed using the 180K AXIOM® SoyaSNP array. Thirteen QTLs related to agronomic traits were mapped to chromosomes 2, 3, 11, 13, 19, and 20, whereas 29 QTLs associated with isoflavone contents were mapped to chromosomes 1, 3, 8, 11, 14, 15, and 17. Notably, the qMGLI11, qMGNI11, qADZI11, and qTI11, which located Gm11_9877690 to Gm11_9955924 interval on chromosome 11, contributed to the high isoflavone contents and explained 11.9% to 20.1% of the phenotypic variation. This QTL region included four candidate genes, encoding β-glucosidases 13, 14, 17-1, and 17-2. We observed significant differences in the expression levels of these genes at various seed developmental stages. Candidate genes within the causal QTLs were functionally characterized based on enriched GO terms and KEGG pathways, as well as the results of a co-expression network analysis. A correlation analysis indicated that certain agronomic traits (e.g., days to flowering, days to maturity, and plant height) are positively correlated with isoflavone content.ConclusionsHerein, we reported that the major QTL associated with isoflavone contents was located in the interval from Gm11_9877690 to Gm11_9955924 (78 kb) on chromosome 11. Four β-glucosidase genes were identified that may be involved in high isoflavone contents of soybean DB-088. Thus, the mutant alleles from soybean DB-088 may be useful for marker-assisted selection in developing soybean lines with high isoflavone contents and superior agronomic traits.

Project description:Soil acidity with associated low nutrient availability is one of the major constraints to soybean production in southwestern Ethiopia. Integrated use of lime and acid-tolerant crops is believed to reduce soil acidity and improve crop production. The experiment was conducted in the field condition of Mettu, southwestern Ethiopia during the 2017/18 main cropping season. The experiment comprised fifteen soybean genotypes and two soil amendment (lime and unlimed) treatments arranged in a split-plot design with three replications. For each treatment, four rows were planted per plot; data related to growth, root, nodule, and yield of the crop were collected at a necessary stage for each. Liming and genotype interaction had significantly (P = 0.01) affected all parameters considered except for hundred seed weight and root volume and were affected only by the main effects of genotypes and liming. A significant reduction for most parameters was found on lime-untreated soil than treated soil. Though some genotypes showed higher performance for root, growth parameters, and yield components under unlimed soils; however, gave higher yield and yield components, when grown on lime-untreated with an average yield reduction of 13.7%, due to soil acidity. The maximum grain yield of (1943.93 kg ha-1) was obtained under lime treated acid soil from PI567046A genotype; while the lowest (510.49 kg ha-1) were recorded from SCS-1genotype under the lime untreated acid soil. Genotype BRS268 showed higher yield (1319.83 kg ha-1) under lime untreated acid soil than lime treated acid soil (1143.47 kg ha-1) and showed less reduction percentage for a number of the nodules, root weight, and number of seeds per plant; while PI567046A showed high reduction percentage for yield, biomass, number of pod and seed per plant. A high difference was observed among the soybean genotypes for soil acidity tolerance, which might be further exploited by breeders for the genetic improvement of soybean. Genotype BRS268 had performed better than other tested genotypes under increased soil acidity. selection would be effective to improve soybean genotypes performance on acid soils and identify low Phosphorus tolerant genotype that helps smallholder farmers optimize soybean productivity on acid soils in the study area. HAWASSA-04 variety is the most tolerant among the tested materials. However, further study is required by considering additional genotypes to reach a conclusive recommendation.

Dataset Information

Isoflavone Malonyltransferases GmIMaT1 and GmIMaT3 Differently Modify Isoflavone Glucosides in Soybean (Glycine max) under Various Stresses.

Publications

Isoflavone Malonyltransferases GmIMaT1 and GmIMaT3 Differently Modify Isoflavone Glucosides in Soybean (<i>Glycine max</i>) under Various Stresses.

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