Unknown

Dataset Information

0

A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates.


ABSTRACT: Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3?L) for rapid (<2?min) and continuous viscosity measurement. By fitting the power-law fluid model to the travel distance of blood through the micro-channel as a function of time and channel configuration, the experimentally acquired blood viscosity was compared with a vacuum-driven capillary viscometer at high shear rates (>500?s-1), at which the power law exponent (n) of zebrafish blood was nearly 1 behaving as a Newtonian fluid. The measured values of whole blood from the micro-channel (4.17cP) and the vacuum method (4.22cP) at 500?s-1 were closely correlated at 27?°C. A calibration curve was established for viscosity as a function of hematocrits to predict a rise and fall in viscosity during embryonic development. Thus, our rapid capillary pressure-driven micro-channel revealed the Newtonian fluid behavior of zebrafish blood at high shear rates and the dynamic viscosity during development.

SUBMITTER: Lee J 

PROVIDER: S-EPMC5434032 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates.

Lee Juhyun J   Chou Tzu-Chieh TC   Kang Dongyang D   Kang Hanul H   Chen Junjie J   Baek Kyung In KI   Wang Wei W   Ding Yichen Y   Carlo Dino Di DD   Tai Yu-Chong YC   Hsiai Tzung K TK  

Scientific reports 20170516 1


Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3μL) for rapid (<2 min) and continuous viscosity measuremen  ...[more]

Similar Datasets

| S-EPMC5997405 | biostudies-literature
| S-EPMC10319024 | biostudies-literature
| S-EPMC6082256 | biostudies-literature
| S-EPMC8517977 | biostudies-literature
| S-EPMC2842211 | biostudies-literature
| S-EPMC9046559 | biostudies-literature
| S-EPMC5332575 | biostudies-literature
| S-EPMC6141135 | biostudies-literature
| S-EPMC2712204 | biostudies-literature
| S-EPMC4405589 | biostudies-literature