Ontology highlight
ABSTRACT:
SUBMITTER: Operario DJ
PROVIDER: S-EPMC5436647 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Operario Darwin J DJ Koeppel Alexander F AF Turner Stephen D SD Bao Yongde Y Pholwat Suporn S Banu Sayera S Foongladda Suporn S Mpagama Stellah S Gratz Jean J Ogarkov Oleg O Zhadova Svetlana S Heysell Scott K SK Houpt Eric R ER
PloS one 20170518 5
Amplicon-based Next Generation Sequencing (NGS) is an emerging method for Mycobacterium tuberculosis drug susceptibility testing (DST) but has not been well described. We examined 158 clinical multidrug-resistant M. tuberculosis isolates via NGS of 11 resistance-associated gene regions covering 3519 nucleotides. Across these gene regions, complete resistance or heteroresistance (defined as 1%-99% mutation) was present in at least one isolate in 6.3% of loci. The number of isolates with heterores ...[more]