Ontology highlight
ABSTRACT:
SUBMITTER: Gao Y
PROVIDER: S-EPMC5436902 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Gao Yuchen Y Xiong Xin X Wong Spencer S Charles Emeric J EJ Lim Wendell A WA Qi Lei S LS
Nature methods 20161024 12
The ability to dynamically manipulate the transcriptome is important for studying how gene networks direct cellular functions and how network perturbations cause disease. Nuclease-dead CRISPR-dCas9 transcriptional regulators, while offering an approach for controlling individual gene expression, remain incapable of dynamically coordinating complex transcriptional events. Here, we describe a flexible dCas9-based platform for chemical-inducible complex gene regulation. From a screen of chemical- a ...[more]