Active Interaction Mapping Reveals the Hierarchical Organization of Autophagy.

ABSTRACT: We have developed a general progressive procedure, Active Interaction Mapping, to guide assembly of the hierarchy of functions encoding any biological system. Using this process, we assemble an ontology of functions comprising autophagy, a central recycling process implicated in numerous diseases. A first-generation model, built from existing gene networks in Saccharomyces, captures most known autophagy components in broad relation to vesicle transport, cell cycle, and stress response. Systematic analysis identifies synthetic-lethal interactions as most informative for further experiments; consequently, we saturate the model with 156,364 such measurements across autophagy-activating conditions. These targeted interactions provide more information about autophagy than all previous datasets,

SUBMITTER: Kramer MH 

PROVIDER: S-EPMC5439305 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature