Correction: Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779.
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ABSTRACT:
SUBMITTER: Vieler A
PROVIDER: S-EPMC5441573 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Publications
Correction: Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779.
Vieler Astrid A Wu Guangxi G Tsai Chia-Hong CH Bullard Blair B Cornish Adam J AJ Harvey Christopher C Reca Ida-Barbara IB Thornburg Chelsea C Achawanantakun Rujira R Buehl Christopher J CJ Campbell Michael S MS Cavalier David D Childs Kevin L KL Clark Teresa J TJ Deshpande Rahul R Erickson Erika E Armenia Ferguson Ann A Handee Witawas W Kong Que Q Li Xiaobo X Liu Bensheng B Lundback Steven S Peng Cheng C Roston Rebecca L RL Sanjaya Simpson Jeffrey P JP TerBush Allan A Warakanont Jaruswan J Zäuner Simone S Farre Eva M EM Hegg Eric L EL Jiang Ning N Kuo Min-Hao MH Lu Yan Y Niyogi Krishna K KK Ohlrogge John J Osteryoung Katherine W KW Shachar-Hill Yair Y Sears Barbara B BB Sun Yanni Y Takahashi Hideki H Yandell Mark M Shiu Shin-Han SH Benning Christoph C
PLoS genetics 20170523 5
[This corrects the article DOI: 10.1371/journal.pgen.1003064.]. ...[more]
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Project description:Unicellular marine algae have promise for providing sustainable and scalable biofuel feedstocks, although no single species has emerged as a preferred organism. Moreover, adequate molecular and genetic resources prerequisite for the rational engineering of marine algal feedstocks are lacking for most candidate species. Heterokonts of the genus Nannochloropsis naturally have high cellular oil content and are already in use for industrial production of high value lipid products. First success in applying reverse genetics makes Nannochloropsis species attractive models to investigate the cell and molecular biology and biochemistry of this fascinating organism group. (Principle findings) Here we present the assembly of the 28.7 Mb genome of Nannochloropsis oceanica CCMP1779. RNA sequencing data from N-replete and N-depleted growth conditions support a total of 11,973 genes, which in addition to automatic annotation were manually inspected to predict the biochemical repertoire for this organism. Among others, more than 100 genes putatively related to lipid metabolism, 114 predicted transcription factors and 109 transcriptional regulators were annotated. In addition, we provide protocols for the transformation of the sequenced strain. (Significance) The availability of genomic and transcriptomic data for Nannochloropsis oceanica CCMP1779, along with efficient transformation protocols provides a blueprint for future detailed gene functional analysis and phylogenetic comparison of Nannochloropsis species by a growing academic community focused on this genus. one sample each of nitrogen-replete and nitrogen-depleted conditions
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Project description:Unicellular marine algae have promise for providing sustainable and scalable biofuel feedstocks, although no single species has emerged as a preferred organism. Moreover, adequate molecular and genetic resources prerequisite for the rational engineering of marine algal feedstocks are lacking for most candidate species. Heterokonts of the genus Nannochloropsis naturally have high cellular oil content and are already in use for industrial production of high value lipid products. First success in applying reverse genetics makes Nannochloropsis species attractive models to investigate the cell and molecular biology and biochemistry of this fascinating organism group. (Principle findings) Here we present the assembly of the 28.7 Mb genome of Nannochloropsis oceanica CCMP1779. RNA sequencing data from N-replete and N-depleted growth conditions support a total of 11,973 genes, which in addition to automatic annotation were manually inspected to predict the biochemical repertoire for this organism. Among others, more than 100 genes putatively related to lipid metabolism, 114 predicted transcription factors and 109 transcriptional regulators were annotated. In addition, we provide protocols for the transformation of the sequenced strain. (Significance) The availability of genomic and transcriptomic data for Nannochloropsis oceanica CCMP1779, along with efficient transformation protocols provides a blueprint for future detailed gene functional analysis and phylogenetic comparison of Nannochloropsis species by a growing academic community focused on this genus.
2012-04-04 | GSE36959 | GEO
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