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Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry.

ABSTRACT: Use of a heterobifunctional photoactivatable cross-linker, sulfo-SDA (diazirine), has yielded high-density data that facilitated structure modeling of individual proteins. We expand the photoactivatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone). This further increases the density of photo-cross-linking to a factor of 20× over conventional cross-linking. Importantly, the two different photoactivatable groups display orthogonal directionality, enabling access to different protein regions, unreachable with a single cross-linker.

SUBMITTER: Belsom A

PROVIDER: S-EPMC5441754 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry.

Belsom Adam A Mudd Gemma G Giese Sven S Auer Manfred M Rappsilber Juri J

Analytical chemistry 20170504 10

Use of a heterobifunctional photoactivatable cross-linker, sulfo-SDA (diazirine), has yielded high-density data that facilitated structure modeling of individual proteins. We expand the photoactivatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone). This further increases the density of photo-cross-linking to a factor of 20× over conventional cross-linking. Importantly, the two different photoactivatable groups display orthogonal directionality, enabling access to differ ...[more]

PMID: 28430416

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