Ontology highlight
ABSTRACT:
SUBMITTER: Liu J
PROVIDER: S-EPMC5441872 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Liu Jie J Ede Christopher C Wright William D WD Gore Steven K SK Jenkins Shirin S SS Freudenthal Bret D BD Todd Washington M M Veaute Xavier X Heyer Wolf-Dietrich WD
eLife 20170523
Synthesis-dependent strand annealing (SDSA) is the preferred mode of homologous recombination in somatic cells leading to an obligatory non-crossover outcome, thus avoiding the potential for chromosomal rearrangements and loss of heterozygosity. Genetic analysis identified the Srs2 helicase as a prime candidate to promote SDSA. Here, we demonstrate that Srs2 disrupts D-loops in an ATP-dependent fashion and with a distinct polarity. Specifically, we partly reconstitute the SDSA pathway using Rad5 ...[more]