Automated deep-phenotyping of the vertebrate brain.
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ABSTRACT:
SUBMITTER: Allalou A
PROVIDER: S-EPMC5441873 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Publications
Automated deep-phenotyping of the vertebrate brain.
Allalou Amin A Wu Yuelong Y Ghannad-Rezaie Mostafa M Eimon Peter M PM Yanik Mehmet Fatih MF
eLife 20170413
Here, we describe an automated platform suitable for large-scale deep-phenotyping of zebrafish mutant lines, which uses optical projection tomography to rapidly image brain-specific gene expression patterns in 3D at cellular resolution. Registration algorithms and correlation analysis are then used to compare 3D expression patterns, to automatically detect all statistically significant alterations in mutants, and to map them onto a brain atlas. Automated deep-phenotyping of a mutation in the mas ...[more]
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