Ontology highlight
ABSTRACT:
SUBMITTER: Lin Y
PROVIDER: S-EPMC5442822 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Lin Yongshun Y Linask Kaari L KL Mallon Barbara B Johnson Kory K Klein Michael M Beers Jeanette J Xie Wen W Du Yubin Y Liu Chengyu C Lai Yinzhi Y Zou Jizhong J Haigney Mark M Yang Hushan H Rao Mahendra M Chen Guokai G
Stem cells translational medicine 20160902 2
Cardiomyocytes can be differentiated from human pluripotent stem cells (hPSCs) in defined conditions, but efficient and consistent cardiomyocyte differentiation often requires expensive reagents such as B27 supplement or recombinant albumin. Using a chemically defined albumin-free (E8 basal) medium, we identified heparin as a novel factor that significantly promotes cardiomyocyte differentiation efficiency, and developed an efficient method to differentiate hPSCs into cardiomyocytes. The treatme ...[more]