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The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells.

ABSTRACT: The human protein ASPG is an enzyme with a putative antitumor activity. We generated in bacteria and then purified a recombinant GST-ASPG protein that we used to characterize the biochemical and cytotoxic properties of the human ASPG. We demonstrated that ASPG possesses asparaginase and PAF acetylhydrolase activities that depend on a critical threonine residue at position 19. Consistently, ASPG but not its T19A mutant showed cytotoxic activity in K562, NALM-6 and MOLT-4 leukemic cell lines but not in normal cells. Regarding the mechanism of action of ASPG, it was able to induce a significant apoptotic death in K562 cells. Taken together our data suggest that ASPG, combining different enzymatic activities, should be considered a promising anti-cancer agent for inhibiting the growth of leukemia cells.

SUBMITTER: Belviso S

PROVIDER: S-EPMC5443537 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells.

Belviso Stefania S Iuliano Rodolfo R Amato Rosario R Perrotti Nicola N Menniti Miranda M

PloS one 20170524 5

The human protein ASPG is an enzyme with a putative antitumor activity. We generated in bacteria and then purified a recombinant GST-ASPG protein that we used to characterize the biochemical and cytotoxic properties of the human ASPG. We demonstrated that ASPG possesses asparaginase and PAF acetylhydrolase activities that depend on a critical threonine residue at position 19. Consistently, ASPG but not its T19A mutant showed cytotoxic activity in K562, NALM-6 and MOLT-4 leukemic cell lines but n ...[more]

PMID: 28542249

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Dataset Information

The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells.

Publications

The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells.

Similar Datasets

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