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Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival.


ABSTRACT: Salmonella have developed a sophisticated machinery to evade immune clearance and promote survival in the infected cells. Previous studies were mostly focused on either bacteria itself or host cells, the interaction mechanism of host-pathogen awaits further exploration. In the present study, we show that Salmonella can exploit mammalian cell non-classical microRNA processing machinery to further process bacterial small non-coding RNAs into microRNA-like fragments. Sal-1, one such fragment with the highest copy number in the infected cells, is derived from Salmonella 5'-leader of the ribosomal RNA transcript and has a 'stem' structure-containing precursor. Processing of Sal-1 precursors to mature Sal-1 is dependent on host cell Argonaute 2 (AGO2) but not Dicer. Functionally, depleting cellular Sal-1 strongly renders the Salmonella bacteria less resistant to the host defenses both in vitro and in vivo. In conclusion, we demonstrate a novel strategy for Salmonella evading the host immune clearance, in which Salmonella produce microRNA-like functional RNA fragments to establish a microenvironment facilitating bacterial survival.

SUBMITTER: Gu H 

PROVIDER: S-EPMC5443790 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival.

Gu Hongwei H   Zhao Chihao C   Zhang Tianfu T   Liang Hongwei H   Wang Xiao-Ming XM   Pan Yi Y   Chen Xi X   Zhao Quan Q   Li Donghai D   Liu Fenyong F   Zhang Chen-Yu CY   Zen Ke K  

Scientific reports 20170524 1


Salmonella have developed a sophisticated machinery to evade immune clearance and promote survival in the infected cells. Previous studies were mostly focused on either bacteria itself or host cells, the interaction mechanism of host-pathogen awaits further exploration. In the present study, we show that Salmonella can exploit mammalian cell non-classical microRNA processing machinery to further process bacterial small non-coding RNAs into microRNA-like fragments. Sal-1, one such fragment with t  ...[more]

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