HTERT-Immortalized Bone Mesenchymal Stromal Cells Expressing Rat Galanin via a Single Tetracycline-Inducible Lentivirus System.
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ABSTRACT:
SUBMITTER: An K
PROVIDER: S-EPMC5444038 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
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hTERT-Immortalized Bone Mesenchymal Stromal Cells Expressing Rat Galanin via a Single Tetracycline-Inducible Lentivirus System.
An Ke K Liu Hui-Ping HP Zhong Xiao-Long XL Deng David Y B DYB Zhang Jing-Jun JJ Liu Zhi-Heng ZH
Stem cells international 20170511
The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. Here, an hTERT-BMSCs/Tet-on/GAL cell line was constructed using a single Tet-on-inducible lentivirus system, and subsequent experiments demonstrated that the secretion of rat GAL from hTERT-BMSCs/Tet-on/GAL was switched on and off ...[more]
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