Dataset Information

Identification and characterization of the BmCyclin L1-BmCDK11A/B complex in relation to cell cycle regulation.

ABSTRACT: Cyclin proteins are the key regulatory and activity partner of cyclin-dependent kinases (CDKs), which play pivotal regulatory roles in cell cycle progression. In the present study, we identified a Cyclin L1 and 2 CDK11 2 CDK11 splice variants, CDK11A and CDK11B, from silkworm, Bombyx mori. We determined that both Cyclin L1 and CDK11A/B are nuclear proteins, and further investigations were conducted to elucidate their spatiofunctional features. Cyclin L1 forms a complex with CDK11A/B and were co-localized to the nucleus. Moreover, the dimerization of CDK11A and CDK11B and the effects of Cyclin L1 and CDK11A/B on cell cycle regulation were also investigated. Using overexpression or RNA interference experiments, we demonstrated that the abnormal expression of Cyclin L1 and CDK11A/B leads to cell cycle arrest and cell proliferation suppression. Together, these findings indicate that CDK11A/B interacts with Cyclin L1 to regulate the cell cycle.

SUBMITTER: Liu TH

PROVIDER: S-EPMC5444353 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Identification and characterization of the BmCyclin L1-BmCDK11A/B complex in relation to cell cycle regulation.

Liu Tai-Hang TH Wu Yun-Fei YF Dong Xiao-Long XL Pan Cai-Xia CX Du Guo-Yu GY Yang Ji-Gui JG Wang Wei W Bao Xi-Yan XY Chen Peng P Pan Min-Hui MH Lu Cheng C

Cell cycle (Georgetown, Tex.) 20170320 9

Cyclin proteins are the key regulatory and activity partner of cyclin-dependent kinases (CDKs), which play pivotal regulatory roles in cell cycle progression. In the present study, we identified a Cyclin L1 and 2 CDK11 2 CDK11 splice variants, CDK11A and CDK11B, from silkworm, Bombyx mori. We determined that both Cyclin L1 and CDK11A/B are nuclear proteins, and further investigations were conducted to elucidate their spatiofunctional features. Cyclin L1 forms a complex with CDK11A/B and were co- ...[more]

PMID: 28318374

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Dataset Information

Identification and characterization of the BmCyclin L1-BmCDK11A/B complex in relation to cell cycle regulation.

Publications

Identification and characterization of the BmCyclin L1-BmCDK11A/B complex in relation to cell cycle regulation.

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