Dataset Information

Isolation and characterization of phosphate solubilizing bacteria from Western Indian Himalayan soils.

ABSTRACT: Previous studies confirmed the existence of diversified microbial flora in the rhizosphere of Himalayan Red Kidney Bean (RKB) (Phaseolus vulgaris L.). Therefore, fifteen different temperate and subtropical regions of Western Indian Himalaya (WIH) were explored for the isolation of RKB rhizosphere-associated Phosphorus (P) solubilizing bacteria. On the basis of qPCR analysis, three soils, i.e., Munsyari, Kandakhal and Nainital soils were selected for the isolation of P solubilizers. Among 133 isolates, three bacteria viz. Lysinibaccilus macroides ST-30, Pseudomonas palleroniana N-26 and Pseudomonas jessenii MP-1 were selected based on their P solubilization potential. Moreover, in vitro seed germination assay was performed to investigate their effectiveness against four native crops viz. (Cicer arietrinum L.), (Vigna radiata L.), (Pisum sativum L.) and (Zea mays L.). Treated seeds showed significant increase in germination efficiency over their respective controls. The results suggest that Lysinibaccilus macroides ST-30, strain is a potential plant growth-promoting bacterium for chickpea (Cicer arietrinum L.) and, therefore, could be implemented as a low-cost bio-inoculant in hill agriculture system.

SUBMITTER: Tomer S

PROVIDER: S-EPMC5447524 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Isolation and characterization of phosphate solubilizing bacteria from Western Indian Himalayan soils.

Tomer Supriya S Suyal Deep Chandra DC Shukla Anjana A Rajwar Jyoti J Yadav Amit A Shouche Yogesh Y Goel Reeta R

3 Biotech 20170529 2

Previous studies confirmed the existence of diversified microbial flora in the rhizosphere of Himalayan Red Kidney Bean (RKB) (Phaseolus vulgaris L.). Therefore, fifteen different temperate and subtropical regions of Western Indian Himalaya (WIH) were explored for the isolation of RKB rhizosphere-associated Phosphorus (P) solubilizing bacteria. On the basis of qPCR analysis, three soils, i.e., Munsyari, Kandakhal and Nainital soils were selected for the isolation of P solubilizers. Among 133 iso ...[more]

PMID: 28555431

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Project description:Phosphate solubilizing bacteria (PSB) is an advantageous way to supply phosphate (P) to plants. The Mediterranean climate of Morocco, especially the low-lying areas, is semi-arid with nutrient-depleted soils in which small-scale, low-income farmers dominate without access to expensive inorganic fertilizers. However, there is not a wide range of PSBs suitable for various agroecological situations. Furthermore, our understanding of the soil and climatic variables that influence their development is limited. This study aims to examine the impacts of specific environmental factors, such as climate and soil, on the abundance, potential, and diversity of PSBs in four agricultural regions of Morocco. To assess the possible impact of these factors on the P solubilization capacity of PSBs and plant growth-promoting (PGP) traits, we analyzed the soil and climate of each sample studied. Similarly, we tested the P solubilization efficiency of the isolates. The bacteria were isolated in a National Botanical Research Institute's phosphate (NBRIP) agar medium. A total of 51 PSBs were studied in this work. The P-solubilization average of Rock P (RP) and Tricalcium P (TCP) of all strains that were isolated from each of the four regions ranged from 18.69 mg.L-1 to 40.43 mg.L-1 and from 71.71 mg.L-1 to 94.54 mg.L-1, respectively. The PGP traits of the isolated strains are positively correlated with the PSBs abundance and the sample characteristics (soil and climate). The morphological and biochemical characteristics of the strain allowed us to identify around nine different bacterial genera, including Bacillus, Pseudomonas, and Rhizobium. The findings showed that bacterial communities, density, and potency are closely correlated to various edapho-climatic conditions such as temperature, precipitation, soil nutrient status, and soil texture. These findings could be used to improve an effective plant-PSBs system and increase agricultural output by taking into account their specific ecological traits and plant growth mechanisms.

Dataset Information

Isolation and characterization of phosphate solubilizing bacteria from Western Indian Himalayan soils.

Publications

Isolation and characterization of phosphate solubilizing bacteria from Western Indian Himalayan soils.

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