Ontology highlight
ABSTRACT:
SUBMITTER: Liu X
PROVIDER: S-EPMC5448163 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Liu Xue X Gallay Clement C Kjos Morten M Domenech Arnau A Slager Jelle J van Kessel Sebastiaan P SP Knoops Kèvin K Sorg Robin A RA Zhang Jing-Ren JR Veening Jan-Willem JW
Molecular systems biology 20170510 5
Genome-wide screens have discovered a large set of essential genes in the opportunistic human pathogen <i>Streptococcus pneumoniae</i> However, the functions of many essential genes are still unknown, hampering vaccine development and drug discovery. Based on results from transposon sequencing (Tn-seq), we refined the list of essential genes in <i>S. pneumoniae</i> serotype 2 strain D39. Next, we created a knockdown library targeting 348 potentially essential genes by CRISPR interference (CRISPR ...[more]